Xingjie ZAN's Research Group


Group Name

Biological interface research group

Principal Investigator

Dr. Prof. Xingjie Zan
Wenzhou Institute, UCAS
Email: zanxj@wiucas.ac.cn

Education

Research Expereices

Awards

Funding Sources

Research

In recent years, our group has focused on delivering active proteins and developing protein materials for disease treatment and tissue engineering. At present, we has constructed a series of active protein delivery systems, including nanoparticles, micro/nano-vesicles, hydrogels, through 1) the coordinative interaction between polypeptide/protein/natural polymer and metal ions, and 2) the multiple interactions between polypeptide/protein and plant polyphenols. And these systems are applied in treating various diseases and tissue regeneration, including diabetes, ophthalmic related diseases, liver related diseases, skin regeneration, bone related implants, etc.
 

Native protein delivery

Proteins play an important role in the diseases treatment. However, due to the fragility, the protein could be fast degraded by enzymes and lose their biological activity when administrated in traditional way (oral or intramuscular injection). We developed a variety of protein delivery vehicles, studied their effects on protein encapsulation, activity maintenance, responsive release, intracellular delivery, and carried out their therapeutic effects in a different disease models.
Protein delivery vehicles constructed from (a) the interaction between hexahistidine and metal ions and (b) the multiple interaction between plant polyphenol and proteins.

The effect of physicochemical properties on the destiny of nano-medicine

With the development of nanotechnology, nano-medicines (drug loaded nanoparticles) bring light to many previously incurable diseases due to its ability to greatly change the pharmacokinetics. It is the key to control the fate and behavior of nano-medicines in cells and in vivo, which could be largely affected by the physicochemical properties (particle size, shape, mechanical properties and surface chemistry). We concentrated on how these physicochemical properties influence the in vitro and in vivo behaviors of nano-medicine, with the purpose to improve clinical outcomes.

Controlling the fate of stem cells for tissue engineering

Stem cells have low immunogenicity and the ability to differentiate into various phenotypes, and can be expended in vitro, which can solve the problem of insufficient functional cells in tissue engineering and disease treatment. The fate of stem cells can be well controlled in vitro, but, it is difficult to be controlled in vivo. Regulating the properties of implants is the key to control the fate of stem cells in vivo and to the success or failure of implantation. We tried to control the fate of stem cells by regulating the physical and chemical properties (mechanical properties, surface chemistry, etc.) of materials, so as to lay a foundation for its further application in tissue engineering.

Selected publications