吴敏课题组
核酸疫苗课题组长（PI）：吴敏教授国科温州研究院特聘研究员，国家级人才，博士生导师，曾任美国大学终身正教授，系研究生部主任和医学院人体标本库主任。从事呼吸系统细菌感染免疫研究，发现了一系列细菌致病因子及揭示了宿主免疫应答新机制。此外，研究CRISPR的生物学，譬如首次发现用于RNA精准编辑的anti-CRISPR 蛋白，并用III型CRISPR-Cas创制新冠病毒基因药物。曾任国际会议主席和分会主持人，多次受邀学术会议作报告。长期得到多项政府和基金会经费资助，是数个国际杂志的编委，副主编和主编。已在包括Nat Microbiol, Nat Communs, Nature，Sci Signal, Mol Cell, Cell Host Microbe, Cell Rep, Cell Res, PNAS, STTT等杂志上发表研究论文，综述和评论250余篇。参与国家自然基金二审和重点项目评审，美国，英国，加拿大和德国等多个国家基金评审。国际杂志评审（Nat Rev Cell Mol Biol, Nat Microbiol)，担任长江学者和海外优青评审。
课题组常年招收岗位(副，助理）研究员，科研助理，博士后和硕博研究生（miw100@ucas.ac.cn）。
研究方向：
1) 目前高度聚焦研发大流行病毒的mRNA疫苗（包括寻找原创性mRNA修饰位点及递送剂）;
2) 寻找细菌抗噬菌体新基因有可能成为基因编辑的工具;
3) 呼吸道传染性疾病的机理和慢性呼吸病的精准基因治疗。
有关研究论文：
1. Li X, He S, Zhou X…Huang C, & Wu M*. Pseudomonas aeruginosa infection augments inflammation through miR-301b repression of c-Myb-mediated immune activation an d infiltration. Nature Microbiology. 2016;1, Article number: 16132 (2016). (Corresponding author).
2. Wang J, Zhou Y, Zhang M, Wu Y, Wu Q, Su W, Xu M, Wu J, Zhang M, Shuai J, Tang W, Lv J, Wu M, Xia Z. YTHDF1-CLOCK axis contributes to pathogenesis of allergic airway inflammation through LLPS. Cell Reports 2024 Mar 14;43(3):113947. doi: 10.1016/j.celrep.2024.113947. (Co-corresponding author).
3. Ma T, Tang Y, Wang T, Yang Y, Zhang Y, Wang R, Zhang Y, Li Y, Wu M, Tang M, Hu X, Zou C, Ren Y, Liu H, Zhang Q, Li H, Wu M, Li J, Zhou X. Chronic pulmonary bacterial infection facilitates breast cancer lung metastasis by recruiting tumor-promoting MHCIIhi neutrophils. Signal Transduct Target Ther. 2023 Aug 11;8(1):296. doi: 10.1038/s41392-023-01542-0.
4. Yan Y, Li R, Chen H, Li Y, Wu M, Wang Z, Yang G. Magnetic nanoagent assisted deciphering of heterogeneous glycans in extracellular vesicles of varied cellular origins. Biosens Bioelectron, 2023 Sep 22;241:115705.doi: 10.1016/j.bios.2023.115705.
5. Li R, Tan S, Yu M, Li X, He S, Wei Y, Jiang JX, an d Wu M. Pseudomonas aeruginosa evades host immunity by Type I CRISPR-Cas mediated endogenous targeting. Cell Research 2016;26(12):1273-1287. PMID: 27857054 doi: 10.1038/cr.2016.135. (with accompany research highlight paper an d cover article with cover image, Corresponding author).
6. Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF. Alternate binding modes of anti-CRISPR viral suppressors AcrF1/2 to Csy surveillance complex revealed by cryo-EM structures. Cell Res. 2017 Jun 2; 27(7):853-864doi: 10.1038/cr.2017.79. PMID: 28574055
7. Chin W*, Zhong G*, Pu Q*, Yang C, Lou W, Florez De Sessions P, Periaswamy B, Liang ZC, Gao S, Chu CW, Bianco S, Bao C, Ding X, Tong YW, Fan W, Wu M$, Hedick J$, Yang YY$. A macromolecular therapeutic approach to eradicate multidrug resistant bacterial infections while mitigating drug resistance onset. Nat Commun 2018 Mar 2;9(1):917. doi: 10.1038/s41467-018-03325-6. ($Co-corresponding author; * co-first authors).
8. Lin P, Pu Q, Wu Q…Wu M*. High-throughput screen reveals sRNAs regulating crRNA biogenesis by targeting CRISPR leader to repress Rho termination. Nat Commun. 2019 Aug 19;10(1):3728. (Corresponding author).
9. Zhou X, Li X, Ye Y, Li Y, Privratsky B, Wang W, Wei Y, an d Wu M. MicroRNA-302b augments host defense to bacteria by regulating inflammatory responses via feedback to the TLR/IRAK4 circuit. Nat Commun 2014;5, Article number: 3619, doi:10.1038/ncomms4619. (Corresponding author). Selected by F1000Prime - special significance in its field by F1000 Faculty. http://f1000.com/prime/718345439
10. Li R, Fang L, Yu M, Li X, Pu Q, Weiland T, Bansal A, Ye SQ, Jiang J an d Wu M. MEG3-4 is a miRNA decoy that regulates IL-1β abundance to initiate an d then limit inflammation to prevent sepsis during lung infection. Science Signaling Jun 26 2018;11(536). pii: eaao2387. doi: 10.1126/scisignal.aao2387. PMID: 29945883 (Corresponding author).
11. Lin P, Guo K, Pu Q,…Jiang J*, Wu M*. Type III CRISPR-Cas for controlling SARS- CoV-2 an d Influenza viruses. Nucl Acid Res. 2022 6;50(8):e47. (Corresponding author).
12. Li L, Shen G, Wu M*, Jiang J*, Xia Q*, Lin P*. CRISPR-Cas-mediated diagnostics. Trends Biotechnol. 2022 May 17:S0167-7799(22)00104-4.
13. Qin S, Tang X, Chen Y, Chen K, Fan N, Xiao W, Zheng Q, Li G, Teng Y, Wu M*, Song X*. mRNA- based therapeutics: powerful an d versatile tools to combat diseases. Signal Transduct Target Ther. 2022 May 21;7(1):166.
14. Qin S, Xiao W, Zhou C…. & Wu M*. Pseudomonas aeruginosa: Pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances an d emerging therapeutics. Signal Transduct Target Ther. 2022, 7(1): 199. (Corresponding author).
15. Pu Q, Guo K, Lin P…Wu M*. Bitter receptor TAS2R138 facilitates lipid droplet degradation in neutrophils during Pseudomonas aeruginosa infection. Signal Transduct Target Ther. 2021 Jun 4;6(1):210. (Corresponding author).
16. Tie Y, Zheng H, He Z…Wu M*, Wei X*. Targeting folate receptor β positive tumor-associated macrophages in lung cancer with a folate-modified liposomal complex. Signal Transduct Target Ther. 2020 Jan 22;5(1):6.
17. Li H, Yang Y, Hong W, Huang M, Wu M*, Zhao X*. Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances an d prospects. Signal Transduct Target Ther. 2020 Jan 3;5(1):1. (Co-corresponding author).
18. Yuan Y, Liao H, Pu Q…Wu M*, Liu L*, Zhu W*. miR-410 induces both epithelial-mesenchymal transition an d radioresistance through activation of the PI3K/mTOR pathway in non-small cell lung cancer. Signal Transduct Target Ther. 2020 Jun 12;5(1):85.
19. Wang Z, Guo K, Gao P…Wu M*. Microbial an d genetic-based framework identifies drug targets in inflammatory bowel disease. Theranostics. 2021 Jun 1;11(15):7491-7506.
20. Wang Z, Guo K, Liu Y, Huang C, Wu M*. Dynamic impact of virome on colitis an d colorectal cancer: Immunity, inflammation, prevention an d treatment. Semin Cancer Biol. 2022 Nov;86(Pt 2):943-954.
21. Wang K, Zhang T, Lei Y…Wu M*, Huang C*, Wei Y*. Identification of ANXA2 (annexin A2) as a specific bleomycin target to induce pulmonary fibrosis by impeding TFEB-mediated autophagic flux. Autophagy. 2018;14(2):269-282. (Corresponding author).
22. Li X, He S, Ye Y, Zhou X, Tan S, Jundt M.C., Lenz C, Lichter N, Hidebrand A, Wang Y, Li G, Huang C, an d Wu M. Lyn delivers bacterial substance to lysosomes for eradication through TLR-2-initiated an d autophagy-mediated phagocytosis. PLoS Pathogens 2016 Jan 6;12(1):e1005363. doi: 10.1371/journal.ppat.1005363. (Corresponding author).
23. Zhao J, Yu X, Zhu M, Kang H, Ma J, Wu M, Gan J, Deng X, an d Liang H. Structural an d molecular mechanism of CdpR involved in quorum-sensing an d bacterial virulence in Pseudomonas aeruginosa. PLOS Biol 2016 Apr 27;14(4):e1002449. doi: 10.1371/journal.pbio.1002449.
24. Yang J, Wang W, Chen Z…Wu M…Wei X*. A vaccine targeting the RBD of the S protein of SARS- CoV-2 induces protective immunity. Nature. 2020 Oct;586(7830):572-577.
25. Deng X, Weerapana E, Ulanovskaya O, Sun F, Liang H, Ji Q, Ye Y, Fu Y, Zhou L, Li J, Zhang H, Wang C, Alvarez S, Hicks LM, Lan L, Wu M, Cravatt BF, He C. Proteome-wide quantification an d characterization of oxidation-sensitive cysteines in pathogenic bacteria. Cell Host Microbe. 2013;13(3):358-70 (most accessed for the first month, featured article for the journal).( http://www.sciencedirect.com/science/article/pii/S193131281300070X).
26. Yuan K, Xie K, Fox J III, Zeng H, Gao H, Huang C, an d Wu M. Decreased Levels of miR-224 an d the Passenger Strand of miR-221 Increase MBD2, Suppressing Maspin an d Promoting Colorectal Tumor Growth an d Metastasis in Mice. Gastroenterology 2013; 145(4):853-864. PMID:23770133 (Corresponding author).
1. Li X, He S, Zhou X…Huang C, & Wu M*. Pseudomonas aeruginosa infection augments inflammation through miR-301b repression of c-Myb-mediated immune activation a
2. Wang J, Zhou Y, Zhang M, Wu Y, Wu Q, Su W, Xu M, Wu J, Zhang M, Shuai J, Tang W, Lv J, Wu M, Xia Z. YTHDF1-CLOCK axis contributes to pathogenesis of allergic airway inflammation through LLPS. Cell Reports 2024 Mar 14;43(3):113947. doi: 10.1016/j.celrep.2024.113947. (Co-corresponding author).
3. Ma T, Tang Y, Wang T, Yang Y, Zhang Y, Wang R, Zhang Y, Li Y, Wu M, Tang M, Hu X, Zou C, Ren Y, Liu H, Zhang Q, Li H, Wu M, Li J, Zhou X. Chronic pulmonary bacterial infection facilitates breast cancer lung metastasis by recruiting tumor-promoting MHCIIhi neutrophils. Signal Transduct Target Ther. 2023 Aug 11;8(1):296. doi: 10.1038/s41392-023-01542-0.
4. Yan Y, Li R, Chen H, Li Y, Wu M, Wang Z, Yang G. Magnetic nanoagent assisted deciphering of heterogeneous glycans in extracellular vesicles of varied cellular origins. Biosens Bioelectron, 2023 Sep 22;241:115705.doi: 10.1016/j.bios.2023.115705.
5. Li R, Tan S, Yu M, Li X, He S, Wei Y, Jiang JX, a
6. Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF. Alternate binding modes of anti-CRISPR viral suppressors AcrF1/2 to Csy surveillance complex revealed by cryo-EM structures. Cell Res. 2017 Jun 2; 27(7):853-864doi: 10.1038/cr.2017.79. PMID: 28574055
7. Chin W*, Zhong G*, Pu Q*, Yang C, Lou W, Florez De Sessions P, Periaswamy B, Liang ZC, Gao S, Chu CW, Bianco S, Bao C, Ding X, Tong YW, Fan W, Wu M$, Hedick J$, Yang YY$. A macromolecular therapeutic approach to eradicate multidrug resistant bacterial infections while mitigating drug resistance onset. Nat Commun 2018 Mar 2;9(1):917. doi: 10.1038/s41467-018-03325-6. ($Co-corresponding author; * co-first authors).
8. Lin P, Pu Q, Wu Q…Wu M*. High-throughput screen reveals sRNAs regulating crRNA biogenesis by targeting CRISPR leader to repress Rho termination. Nat Commun. 2019 Aug 19;10(1):3728. (Corresponding author).
9. Zhou X, Li X, Ye Y, Li Y, Privratsky B, Wang W, Wei Y, a
10. Li R, Fang L, Yu M, Li X, Pu Q, Weiland T, Bansal A, Ye SQ, Jiang J a
11. Lin P, Guo K, Pu Q,…Jiang J*, Wu M*. Type III CRISPR-Cas for controlling SARS- CoV-2 a
12. Li L, Shen G, Wu M*, Jiang J*, Xia Q*, Lin P*. CRISPR-Cas-mediated diagnostics. Trends Biotechnol. 2022 May 17:S0167-7799(22)00104-4.
13. Qin S, Tang X, Chen Y, Chen K, Fan N, Xiao W, Zheng Q, Li G, Teng Y, Wu M*, Song X*. mRNA- based therapeutics: powerful a
14. Qin S, Xiao W, Zhou C…. & Wu M*. Pseudomonas aeruginosa: Pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances a
15. Pu Q, Guo K, Lin P…Wu M*. Bitter receptor TAS2R138 facilitates lipid droplet degradation in neutrophils during Pseudomonas aeruginosa infection. Signal Transduct Target Ther. 2021 Jun 4;6(1):210. (Corresponding author).
16. Tie Y, Zheng H, He Z…Wu M*, Wei X*. Targeting folate receptor β positive tumor-associated macrophages in lung cancer with a folate-modified liposomal complex. Signal Transduct Target Ther. 2020 Jan 22;5(1):6.
17. Li H, Yang Y, Hong W, Huang M, Wu M*, Zhao X*. Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances a
18. Yuan Y, Liao H, Pu Q…Wu M*, Liu L*, Zhu W*. miR-410 induces both epithelial-mesenchymal transition a
19. Wang Z, Guo K, Gao P…Wu M*. Microbial a
20. Wang Z, Guo K, Liu Y, Huang C, Wu M*. Dynamic impact of virome on colitis a
21. Wang K, Zhang T, Lei Y…Wu M*, Huang C*, Wei Y*. Identification of ANXA2 (annexin A2) as a specific bleomycin target to induce pulmonary fibrosis by impeding TFEB-mediated autophagic flux. Autophagy. 2018;14(2):269-282. (Corresponding author).
22. Li X, He S, Ye Y, Zhou X, Tan S, Jundt M.C., Lenz C, Lichter N, Hidebrand A, Wang Y, Li G, Huang C, a
23. Zhao J, Yu X, Zhu M, Kang H, Ma J, Wu M, Gan J, Deng X, a
24. Yang J, Wang W, Chen Z…Wu M…Wei X*. A vaccine targeting the RBD of the S protein of SARS- CoV-2 induces protective immunity. Nature. 2020 Oct;586(7830):572-577.
25. Deng X, Weerapana E, Ulanovskaya O, Sun F, Liang H, Ji Q, Ye Y, Fu Y, Zhou L, Li J, Zhang H, Wang C, Alvarez S, Hicks LM, Lan L, Wu M, Cravatt BF, He C. Proteome-wide quantification a
26. Yuan K, Xie K, Fox J III, Zeng H, Gao H, Huang C, a
