报告题目：How oligomerization state dictates the cleavage activity of prokaryotic Argonaute?
报告时间：2024年12月19日（周四） 下午14：30
报告地点：3号楼307会议室
报告人：李壮 副教授
邀请人：吴敏 研究员
简介：湖北大学生命科学学院副教授，湖北省“百人计划”创新人才。自2022年正式成立实验室以来，主要开展可编程核酸酶的基因挖掘、生化性质以及工作机理的探究，目前实验室代表性的工作包括对原核Argonaute的研究 (Molecular Cell 2024, Cell Research 2023)、Cas13h的工作机理研究 (Nucleic Acids Res. 2024)、I型CRISPR系统Cascade变体复合物的研究 (Molecular Cell 2024)等。
摘要：The prokaryotic Argonaute nucleases contribute to host defense and represent promising biotechnology tools. My first story is about PfAgo, an Argonaute from the thermophilic archaeon Pyrococcus furiosus. We found the target DNA-induced dimerization mechanism for PfAgo and observed similar dimerization configurations in another two closely related Argonaute. Mostly importantly, we found that dimerization-mediated stabilization of catalytic loops is important for target DNA cleavage activity. My second story is about SPARDA, a short prokaryotic Argonaute that exhibits collateral nuclease activity. We found that the target DNA-induced filament formation is critical for its activity. Altogether, these two stories highlighted how the oligomerization states dictate the nuclease activity of prokaryotic Argonaute.
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